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Human Antithrombin is derived from human plasma, purified through affinity chromatography and gel filtration chromatography methods, and subjected to S/D virus inactivation steps.
Human Antithrombin is a serine protease inhibitor that forms a complex with heparin, leading to the inactivation of factor IIa (thrombin) and factor Xa. The biochemical potency of heparin can be determined based on the catalytic dissociation rate of residual coagulation factors on the corresponding chromogenic substrates.
Company Name :
Beijing Asnail Biotechnology Co.LtdBrand :
AsnailFounded in 2017, Beijing Asrael Biotechnology Co., Ltd. has a business scope that includes the R&D and production of laboratory instrument consumables, animal blood products and product development in the diagnostic reagent industry.
In the direction of laboratory instrument consumables, for the three industrial directions of biotechnology and pharmaceutical industry, thrombosis and hemostasis industry and automotive polymer materials industry, Asrael screens out the corresponding combination of instruments and equipment, reagent consumables.
We adhere to the business philosophy of "professional first, honesty and trustworthiness", provide professional services and high-quality products for customers in related fields, shorten the process of customer procurement, improve efficiency, save costs, and strive to become a stable supplier trusted by customers.
Asrael's business objectives are:
We do not seek to be the largest in the industry, but rather to be the one that endures the longest.
We do not seek the fastest cooperation with clients, but rather the most enduring.
①Heparinases
Heparinases can clave glycosidic bonds of heparin and/or heparan sulfate by a β-elimination mechanism, generating unsaturated products (mostly
disaccharides) with a double bond be-tween C4 and C5 of the uronate residue.
Features:
● Natural heparinase, from Flavobacterium heparinum.
● It has the highest specific activity compare with other products on the market.
● Pure enzyme, free of BSA or other protein impurities.
● Good stability, consistent batch reproducibility and degradation characteristics.
②TOSOH TSKgel SWXL Columns
Tosoh SWxl series columns feature high porous volume/unit volume ratio, low sample absorption, and high column efficiency. Could be used for MW
determinations of heparin and LMWHs.
③BioCore™ SEC columns
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution. The column is durable and can provide
better volume exclusion separation.
④Dionex™ IonPac™ AS11 IC Columns
The Column specifically designed to resolve a large number of inorganic anions and organicacid anions from a single sample injection in one gradient run using hydroxide eluent systems.USP officially specifies the method for the identification of heparin sodium chromatography.
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Category
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Content
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Origin
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China
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Brand
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Asni
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Product Code
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AS00-0132
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Application
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For affinity chromatography of anticoagulant thrombin, separation and purification of proteins, carbohydrates and other substances; research on protein-carbohydrate interactions; development of characterization and diagnostic tools based on these interactions.
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Packaging
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10 IU
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CAS Number
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9000-94-6
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Purity
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Specific activity: ≥6 IU/mg%
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Imported
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No
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Form & Composition
Human antithrombin is lyophilized.
Main Ingredients: Human antithrombin 10 IU
Filler: TRIS 60.5 mg
Physical and chemical properties
Appearance: White lyophilized powder
Purity: Electrophoresis pure
Molecular weight: approx. 56 kDa
Percent absorbance coefficient (l = 1 cm, λ = 280 nm, 1%): about 10
Specific activity: ≥6 IU/mg
How to use
According to the application requirements, an appropriate amount of water for injection or buffer solution was added to obtain the required concentration of human antithrombin solution.
[Stability and storage methods]
The lyophilized human antithrombin is stored at -20 °C and has an expiration date of more than 12 months;
1 IU/mL human antithrombin solution should be stored at 2-8°C protected from light and had an expiration date of more than 1 month.
Precautions
Human antithrombin is mainly used to detect the anti-FIIa activity and anti-FXa activity of anticoagulant drugs such as heparin, and can only be used for laboratory scientific research, in vitro diagnosis and industrial quality control, and must not be used as a drug or in vivo diagnosis.
This reagent is not intended as a standard.
Application examples
Human antithrombin has a wide range of uses, such as:
The ratio of anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin was measured
The anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin were accurately detected
It is used for antithrombin affinity chromatography to separate and purify proteins, sugars and other substances
Study protein-carbohydrate interactions and develop characterization and diagnostic tools based on them
Ordering Information
Catalog number: AS00-0132
Package size: 10 IU
Delivery: In stock
About the Hair Color Substrate Method
Human antithrombin is commonly used to test the potency of heparin (chromogenic substrate method).
chromogenic substrates are chemically synthesized small peptides with a chromogenic group (pNA) at one end. Specific enzymes, such as thrombin, catalyze the separation of pNA. Free pNA can be detected by spectrophotometer or microplate reader to determine the activity of the corresponding substance.
Common chromogenic substrates are: thrombin pigment substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.
Chromogenic assays are currently the main heparin titer detection method, which is the detection method of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP), and will be included in the 2015 edition of the Chinese Pharmacopoeia. This method is also widely used in laboratory studies and in vitro diagnostics.
By controlling the conditions of the reaction system, the chromogenic substrate method can detect coagulation factors, cofactors, or inhibitors of coagulation factors.
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