Form & Composition

S-2238 is lyophilized.

Main ingredients: S-2238 10 mg, about 15 μmol;

Filler: mannitol 120 mg

Physical and chemical properties

CAS Registry Number: 113711-77-6

Molecular Formula: H-D-Phe-Pip-Arg-pNA·2HCl

Molecular weight: 625.6 Da

Purity: ≥ 98% (HPLC)

Absorbance coefficient (405 nm): ≤0.120 [L/(g·cm)]

Solubility: > 10 mg/ml in purified water

How to use

According to the application requirements, an appropriate amount of water for injection or buffer solution was added to obtain the required concentration of S-2238 solution.

Stability and storage method

S-2238 lyophilized product should be stored at 2-8°C in the dark, and the expiration date should be more than 24 months;

3 mmol/L S-2238 solution was stored at 2-8°C in the dark, and the expiration date was more than 1 month.

Precautions

S-2238 is intended for use in the detection of heparin anti-FIIa activity and should only be used for laboratory scientific research, in vitro diagnostics and industrial quality control, and should not be used as a drug or in vivo diagnosis.

Application examples

The thrombin chromogenic substrate S-2238 has a wide range of uses, such as:

The anti-FXa activity and anti-FIIa activity ratio of unfractionated heparin and low molecular weight heparin were tested

Accurate detection of anti-FIIa activity of unfractionated heparin and low molecular weight heparin

To evaluate the anti-FIIa activity of chemically synthesized substances or tissue extracts

The concentration of antithrombin in a blood sample is measured to diagnose antithrombin deficiency

The concentration of heparin in the blood is monitored during the procedure

Production of kits for automated hematology analyzers

Ordering Information

Catalog number: AS00-0101

Packing: 25mg

Delivery: In stock

About the Hair Color Substrate Method

chromogenic substrates are chemically synthesized small peptides with a chromogenic group (pNA) at one end. Specific enzymes, such as thrombin, catalyze the separation of pNA. Free pNA can be detected by spectrophotometer or microplate reader to determine the activity of the corresponding substance.

Common chromogenic substrates are: thrombin pigment substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.

Chromogenic assays are the main heparin titer detection methods, which are prescribed by the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP) for the detection of heparin (especially low molecular weight heparin). This method is also widely used in laboratory studies and in vitro diagnostics.

By controlling the conditions of the reaction system, the chromogenic substrate method can detect coagulation factors, cofactors, or inhibitors of coagulation factors.