Form & Composition

Human antithrombin is lyophilized.

Main Ingredients: Human antithrombin 10 IU

Filler: TRIS 60.5 mg

Physical and chemical properties

Appearance: White lyophilized powder

Purity: Electrophoresis pure

Molecular weight: approx. 56 kDa

Percent absorbance coefficient (l = 1 cm, λ = 280 nm, 1%): about 10

Specific activity: ≥6 IU/mg

How to use

According to the application requirements, an appropriate amount of water for injection or buffer solution was added to obtain the required concentration of human antithrombin solution.

[Stability and storage methods]

The lyophilized human antithrombin is stored at -20 °C and has an expiration date of more than 12 months;

1 IU/mL human antithrombin solution should be stored at 2-8°C protected from light and had an expiration date of more than 1 month.

Precautions

Human antithrombin is mainly used to detect the anti-FIIa activity and anti-FXa activity of anticoagulant drugs such as heparin, and can only be used for laboratory scientific research, in vitro diagnosis and industrial quality control, and must not be used as a drug or in vivo diagnosis.

This reagent is not intended as a standard.

Application examples

Human antithrombin has a wide range of uses, such as:

The ratio of anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin was measured

The anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin were accurately detected

It is used for antithrombin affinity chromatography to separate and purify proteins, sugars and other substances

Study protein-carbohydrate interactions and develop characterization and diagnostic tools based on them

Ordering Information

Catalog number: AS00-0132

Package size: 10 IU

Delivery: In stock

About the Hair Color Substrate Method

Human antithrombin is commonly used to test the potency of heparin (chromogenic substrate method).

chromogenic substrates are chemically synthesized small peptides with a chromogenic group (pNA) at one end. Specific enzymes, such as thrombin, catalyze the separation of pNA. Free pNA can be detected by spectrophotometer or microplate reader to determine the activity of the corresponding substance.

Common chromogenic substrates are: thrombin pigment substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.

Chromogenic assays are currently the main heparin titer detection method, which is the detection method of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP), and will be included in the 2015 edition of the Chinese Pharmacopoeia. This method is also widely used in laboratory studies and in vitro diagnostics.

By controlling the conditions of the reaction system, the chromogenic substrate method can detect coagulation factors, cofactors, or inhibitors of coagulation factors.