Characteristics of Heparinase I

Heparinase I produced by Beijing Asriel Biotechnology Co., Ltd. has the following characteristics:

Natural heparinase, derived from native heparin-degrading bacteria

Exhibits high specific activity (417 IU/mg)

Pure enzyme, free from BSA or other impurity proteins

Good stability, with consistent batch-to-batch reproducibility and degradation characteristics.

The form and composition of Heparinase I

Heparinase I is in solution form.

Main component: Heparinase I

Buffer solution: Phosphate buffer solution

The properties and specifications of Heparinase I

Name: Heparinase I; heparin lyase I; heparin eliminase I

Source: Flavobacterium heparinum

EC Number: 4.2.2.7

CAS Number: 9025-39-2

MDL Number: MFCD00131317

Molecular Weight: 42.8 kDa

Purity: ≥ 90% (HPLC)

Solubility: Soluble in pure water

Specific Activity: > 400 IU/mg

Concentration: 10 IU/mL

Mechanism of action, substrate specificity and unit definition of heparinase I

Mechanism of action of heparinase I: Heparinase selectively cleaves the α(1-4) glycosidic bond between glucosamine and uronic acid in sulfated heparin-glycans. This shearing process is accomplished by an elimination reaction to produce oligosaccharides containing unsaturated uronic acid residues (double bonds located between C4 and C5). The degradation products can be detected by UV spectroscopy (232 nm).

Substrate specificity of heparinase I: Heparin, heparan sulfate (relative activity approx. 3:1)

Definition of active units of heparinase I: 1 unit (1 IU) of heparinase I produces 1 μmol of unsaturated uronic acid per minute at pH 7.0 and 30 °C. 1 IU is approximately equal to 600 un (Sigma units).

How to use heparinase I

According to the application requirements, an appropriate amount of buffer solution was added to obtain the required concentration of heparinase I solution.

Stability and storage method of heparinase I

Heparinase I is stored at -20 °C in the dark, and the expiration date is about 12 months;

Leave the diluted heparinase I at -20 °C to prevent contamination.

Heparinase I is transported over long distances and requires the use of dry ice as a refrigerant.

Precautions for heparinase I

Heparinase I is suitable for the degradation of heparin and heparan sulfate and can only be used for laboratory scientific research, in vitro diagnostics and industrial quality control, and should not be used as a drug or in vivo diagnosis.

Heparinase application example

Heparinase has a wide range of scientific and industrial uses in scientific research and industry, such as:

Degrade heparin for structural analysis, such as enoxaparin sodium 1,6-ring formation rate test, heparin sodium disaccharide profiling

Heparin is partially degraded to obtain low molecular weight heparin, which is chemically modified or grafted to other materials

Degradation of crude heparin to detect its source (qPCR)

Degrade heparin and heparan sulfate to prepare heparin disaccharides and heparin oligosaccharides

Processing of plasma or other tissues for further study

Large-scale production of tinzaparin

Heparinase I, heparanase II, and heparinase III are commonly used together.

Heparinase I References

1. Linker, A., and Hovingh, P. Meth. Enzymol. 28, 902, (1972)

2. Lohse, D.L., and Linhardt, R.J. J. Biol. Chem. 267, 24347, (1992)

About heparin and heparan sulfate

Heparin and heparan sulfate are glycosaminoglycans (GAGs), which are linear polysaccharide mixtures linked by a repeating disaccharide unit composed of a uronic acid (D-glucuronic acid or L-iduronic acid) and a D-glucosamine/N-acetylglucosamine repeating disaccharide unit. Varying degrees of sulfation can occur on each monosaccharide residue, which can contain zero to three sulfate groups. In general, heparan is less sulfated than heparin.

Heparin and its derivative, low-molecular-weight heparin, are widely used anticoagulants. Heparinase plays an important role in the research and production of heparin and low molecular weight heparin.