Form & Composition

Activated factor X is lyophilized.

Main Ingredients: Activated X-factor 71nkat

Filler: TRIS 60.5 mg

Physical and chemical properties

Appearance: White lyophilized powder

Purity: Electrophoresis pure

Molecular weight: Approx. 44 kDa

Percent absorbance coefficient (l=1 cm, λ=280 nm, 1%): approx. 12

Specific activity: ≥90 IU/mg

How to use

According to the application requirements, an appropriate amount of water for injection or buffer solution was added to obtain the required concentration of activated factor X solution.

[Stability and storage methods]

The activated X-factor lyophilized product was stored at -20°C with an expiration date of more than 12 months;

0.25 IU/ml Activated Factor X Solution Store at 2-8 °C protected from light and have an expiration date of approximately 1 month.

Precautions

Activated factor X is mainly used to detect the anti-FXa titer of heparin, and can only be used for laboratory scientific research, in vitro diagnostics and industrial quality control, and should not be used as a drug or in vivo diagnosis.

Application examples

Activated X-Factor has a wide range of uses, such as:

The ratio of anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin was measured

Accurate detection of anti-FXa activity of unfractionated heparin and low molecular weight heparin

Monitor the amount of heparin in blood samples

The amount of antithrombin in the blood is measured to diagnose antithrombin deficiency

Ordering Information

Activated X-factor (FXa) is sold by bottle.

Order No.: AS00-0121

Package specifications: 71NKAT

Delivery: In stock

About the Hair Color Substrate Method

Activated factor X (FXa) can be used to detect the anti-FXa activity of heparin (chromogenic substrate method).

The chromogenic substrate is a chemically synthesized small peptide with a chromophore (pNA) at one end. Specific enzymes, such as thrombin, catalyze the separation of pNA. Free pNA can be detected by spectrophotometer or microplate reader to determine the activity of the corresponding substance.

Common chromogenic substrates are: thrombin pigment substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.

The chromogenic substrate method is currently the main method for testing the potency of heparin, which is the detection method of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP). This method is also widely used in laboratory studies and in vitro diagnostics.

By controlling the conditions of the reaction system, the chromogenic substrate method can detect coagulation factors, cofactors, or inhibitors of coagulation factors.