EN
RU
The Asnail™ Heparin Anti-Factor 10 Assay is a two-step chromogenic substrate assay for the detection of heparin activity (including unfractionated heparin and low molecular weight heparin) in manual or automated experiments. This method is only suitable for the detection of heparin in human citric acid plasma or purified solution.
The kit is intended for scientific research only and is prohibited for clinical diagnosis and treatment.
Company Name :
Beijing Asnail Biotechnology Co.LtdBrand :
AsnailFounded in 2017, Beijing Asrael Biotechnology Co., Ltd. has a business scope that includes the R&D and production of laboratory instrument consumables, animal blood products and product development in the diagnostic reagent industry.
In the direction of laboratory instrument consumables, for the three industrial directions of biotechnology and pharmaceutical industry, thrombosis and hemostasis industry and automotive polymer materials industry, Asrael screens out the corresponding combination of instruments and equipment, reagent consumables.
We adhere to the business philosophy of "professional first, honesty and trustworthiness", provide professional services and high-quality products for customers in related fields, shorten the process of customer procurement, improve efficiency, save costs, and strive to become a stable supplier trusted by customers.
Asrael's business objectives are:
We do not seek to be the largest in the industry, but rather to be the one that endures the longest.
We do not seek the fastest cooperation with clients, but rather the most enduring.
①Heparinases
Heparinases can clave glycosidic bonds of heparin and/or heparan sulfate by a β-elimination mechanism, generating unsaturated products (mostly
disaccharides) with a double bond be-tween C4 and C5 of the uronate residue.
Features:
● Natural heparinase, from Flavobacterium heparinum.
● It has the highest specific activity compare with other products on the market.
● Pure enzyme, free of BSA or other protein impurities.
● Good stability, consistent batch reproducibility and degradation characteristics.
②TOSOH TSKgel SWXL Columns
Tosoh SWxl series columns feature high porous volume/unit volume ratio, low sample absorption, and high column efficiency. Could be used for MW
determinations of heparin and LMWHs.
③BioCore™ SEC columns
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution. The column is durable and can provide
better volume exclusion separation.
④Dionex™ IonPac™ AS11 IC Columns
The Column specifically designed to resolve a large number of inorganic anions and organicacid anions from a single sample injection in one gradient run using hydroxide eluent systems.USP officially specifies the method for the identification of heparin sodium chromatography.
illustrate
Heparin is a sulfated polysaccharide that has a high affinity for thrombin. When combined with heparin, antithrombin exhibits rapid and effective inhibitory activity against coagulation factors such as serine proteases IXa, Xa, and thrombin. Low-molecular-weight heparin (LMWH) and heparin analogues, such as dalteparin sodium, inhibit factor Xa activity more effectively than thrombin activity, while unfractionated heparin (UFH) effectively inhibits thrombin and other serine protease activity. Pentosaccharide inhibits Xa factor more specifically.
This heparin test is a two-step anti-decatoactivity assay for accurate and sensitive determination of heparin concentrations in plasma or purified solutions. The plasma of the test sample needs to be diluted before analysis.
The purified human thrombin used in the experiment is predominantly present in the α form (obtained by direct activation of prothrombin) and exhibits higher coagulation reactivity than the "degraded" β or γ form under the condition of an equivalent concentration of chromogenic substrate.
The assay uses antithrombin and factor Xa reagents pre-diluted in specific buffers (not provided in this kit) and is compliant with the United States Pharmacopeia (USP) and the European Pharmacopoeia (EP).
Reagent composition
R1: Reagent 1 : Antithrombin III (human)
Antithrombin III (human), lyophilized with approximately 5 IU of activity per vial, containing BSA.
2 bottles (10mL bottle size).
R2: Reagent 2 : Activated Ten Factor (Bovine Source)
Activated 10-factor (bovine derived) lyophilized product containing approximately 40 μg (i.e., approximately 90 nkats, measured under optimized conditions for S-2765-specific substrate) activity per vial. Activated ten-factor concentrations are precisely adjusted for each lot to provide optimized assay reactivity and linearity.
2 bottles (10mL bottle size).
R3: Reagent 3: Ten-factor-specific chromogenic substrate S-2765
Ten-factor-specific chromogenic substrate S-2765, each vial contains approximately 4 mg (approximately 6 μmol) of chromogenic substrate lyophilized product, containing mannitol.
2 bottles (10mL bottle size).
Public Account
IPv6 network supported
Product categories
Technical Articles
