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Activated Factor X (FXa) is the product of calf plasma Factor X activation by Factor X activator of Luceri viper, purified by ion exchange and gel filtration chromatography, and added to the S/D virus inactivation step. FXa works with other blood factors in the body to activate prothrombin to produce thrombin, triggering a coagulation waterfall reaction.
Company Name :
Beijing Asnail Biotechnology Co.LtdBrand :
AsnailFounded in 2017, Beijing Asrael Biotechnology Co., Ltd. has a business scope that includes the R&D and production of laboratory instrument consumables, animal blood products and product development in the diagnostic reagent industry.
In the direction of laboratory instrument consumables, for the three industrial directions of biotechnology and pharmaceutical industry, thrombosis and hemostasis industry and automotive polymer materials industry, Asrael screens out the corresponding combination of instruments and equipment, reagent consumables.
We adhere to the business philosophy of "professional first, honesty and trustworthiness", provide professional services and high-quality products for customers in related fields, shorten the process of customer procurement, improve efficiency, save costs, and strive to become a stable supplier trusted by customers.
Asrael's business objectives are:
We do not seek to be the largest in the industry, but rather to be the one that endures the longest.
We do not seek the fastest cooperation with clients, but rather the most enduring.
①Heparinases
Heparinases can clave glycosidic bonds of heparin and/or heparan sulfate by a β-elimination mechanism, generating unsaturated products (mostly
disaccharides) with a double bond be-tween C4 and C5 of the uronate residue.
Features:
● Natural heparinase, from Flavobacterium heparinum.
● It has the highest specific activity compare with other products on the market.
● Pure enzyme, free of BSA or other protein impurities.
● Good stability, consistent batch reproducibility and degradation characteristics.
②TOSOH TSKgel SWXL Columns
Tosoh SWxl series columns feature high porous volume/unit volume ratio, low sample absorption, and high column efficiency. Could be used for MW
determinations of heparin and LMWHs.
③BioCore™ SEC columns
Asnail co-developed the BioCore SEC-HP columns, tailored for molecular weight (MW) and MW distribution. The column is durable and can provide
better volume exclusion separation.
④Dionex™ IonPac™ AS11 IC Columns
The Column specifically designed to resolve a large number of inorganic anions and organicacid anions from a single sample injection in one gradient run using hydroxide eluent systems.USP officially specifies the method for the identification of heparin sodium chromatography.
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Product Information
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Details
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Place of Origin
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China
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Brand
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Asnail
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Article Number
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AS00-0101
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Purpose
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Detect the ratio of anti-Flla activity to anti-FXa activity in unfractionated heparin and low-molecular-weight heparin; accurately measure anti-FXa activity in both types of heparin
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Packaging Specification
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71 nkat
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CAS Number
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9002-05-5
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Purity
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99%
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Is it Imported?
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No
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Form & Composition
Activated factor X is lyophilized.
Main Ingredients: Activated X-factor 71nkat
Filler: TRIS 60.5 mg
Physical and chemical properties
Appearance: White lyophilized powder
Purity: Electrophoresis pure
Molecular weight: Approx. 44 kDa
Percent absorbance coefficient (l=1 cm, λ=280 nm, 1%): approx. 12
Specific activity: ≥90 IU/mg
How to use
According to the application requirements, an appropriate amount of water for injection or buffer solution was added to obtain the required concentration of activated factor X solution.
[Stability and storage methods]
The activated X-factor lyophilized product was stored at -20°C with an expiration date of more than 12 months;
0.25 IU/ml Activated Factor X Solution Store at 2-8 °C protected from light and have an expiration date of approximately 1 month.
Precautions
Activated factor X is mainly used to detect the anti-FXa titer of heparin, and can only be used for laboratory scientific research, in vitro diagnostics and industrial quality control, and should not be used as a drug or in vivo diagnosis.
Application examples
Activated X-Factor has a wide range of uses, such as:
The ratio of anti-FIIa activity and anti-FXa activity of unfractionated heparin and low molecular weight heparin was measured
Accurate detection of anti-FXa activity of unfractionated heparin and low molecular weight heparin
Monitor the amount of heparin in blood samples
The amount of antithrombin in the blood is measured to diagnose antithrombin deficiency
Ordering Information
Activated X-factor (FXa) is sold by bottle.
Order No.: AS00-0121
Package specifications: 71NKAT
Delivery: In stock
About the Hair Color Substrate Method
Activated factor X (FXa) can be used to detect the anti-FXa activity of heparin (chromogenic substrate method).
The chromogenic substrate is a chemically synthesized small peptide with a chromophore (pNA) at one end. Specific enzymes, such as thrombin, catalyze the separation of pNA. Free pNA can be detected by spectrophotometer or microplate reader to determine the activity of the corresponding substance.
Common chromogenic substrates are: thrombin pigment substrate S-2238, FXa chromogenic substrate S-2765 and S-2222.
The chromogenic substrate method is currently the main method for testing the potency of heparin, which is the detection method of heparin (especially low molecular weight heparin) specified in the European Pharmacopoeia (EP) and the United States Pharmacopoeia (USP). This method is also widely used in laboratory studies and in vitro diagnostics.
By controlling the conditions of the reaction system, the chromogenic substrate method can detect coagulation factors, cofactors, or inhibitors of coagulation factors.
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